Coding

Part:BBa_K4695011:Design

Designed by: Maciej Małolepszy   Group: iGEM23_IFB-Gdansk   (2023-10-01)


estJ6


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 16
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 16
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 498
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

FLANKED 5' fusion site: AATG, 3' fusion site: AGGT.
Source sequence was optimized and checked for BsaI and SapI restriction sites using: http://genomes.urv.es/OPTIMIZER/

This part has a START codon at the 5' end of the sequence and no STOP codon at the 3' end because it was designed as a part of a fusion protein with interchangable domains. If you want to express only this coding sequence, please add the STOP codon in your design.

Source

Soil metagenomic library
GenBank: https://www.ncbi.nlm.nih.gov/nuccore/MK037455.1/
Article: https://doi.org/10.1016/j.ecoenv.2019.110148

References